First report on the molecular prevalence of Mycoplasma capricolum subspecies capripneumoniae ( Mccp ) in goats the cause of contagious caprine pleuropneumonia (CCPP) in Balochistan province of Pakistan

Contagious caprine pleuropneumonia (CCPP) caused by Mycoplasma capricolum subspecies capripneumoniae ( Mccp ) is a disease of goats which causes high morbidity and mortality and is reported in many countries of the world. There are probably no reports on the molecular prevalence of Mccp , Mycoplasma capricolum subsp . capricolum ( Mcc ) and Mycoplasma putrefaciens ( Mp ) in Balochistan and any other part of Pakistan. Thirty goats ( n = 30) with marked respiratory symptoms were selected and procured from forty goat flocks in Pishin district of Balochistan in 2008. The genomic deoxyribonucleic acid (DNA) from the lung samples ( n = 30) of the slaughtered goats was purified and subjected to polymerase chain reaction (PCR) assays for the presence of Mycoplasma mycoides cluster members and Mp. The PCR-RFLP (restriction fragment length polymorphism) was also used to further confirm the Mccp . Of the thirty lung samples 17 (56.67%) were positive for the molecular prevalence of Mcc , Mccp and Mp . In total the molecular prevalence was observed as 17.65% for Mccp ( n = 3), 70.59% for Mcc ( n = 12) and 11.76% for Mp ( n = 2). The RFLP profile has also validated the PCR results of Mccp by yielding two bands of 190 and 126 bp. The results of PCR-RFLP coupled with the presence of fibrinous pleuropneumonia and pleurisy during postmortem of goats ( n = 3) strongly indicated the prevalence of CCPP in this part of world. Moreover the prevalence of Mcc and Mp is also alarming in the study area. We report for the very first time the molecular prevalence of Mcc , Mccp , and Mp in the lung tissues of goats in the Pishin district of Balochistan, Pakistan.