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C5a As A Model For Chemotactic Factor-Stimulated Tyrosine Phosphorylation In The Human Neutrophil

JOURNAL OF IMMUNOLOGY, no. 5 (1994): 2479-2487

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Abstract

Human neutrophils were exposed to the chemotactic factors C5a, FMLP, IL-8, leukotriene B-4, and PAF, for 30 s, and subsequently analyzed for protein tyrosine phosphorylation by immunoblotting whole cell lysates with a polyclonal antiphosphotyrosine Ab. All chemotactic factors caused the rapid de novo tyrosine phosphorylation of a broad ba...More

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