Analyte capture in microfluidic heterogeneous immunoassays

H G Parsa,Curtis D Chin,Puttisarn Mongkolwisetwara,B W Lee,J J Wang,Samuel K Sia

New York, NY（2010）

引用 0|浏览7

暂无评分

摘要

Despite the prevalence of microfluidic-based heterogeneous immunoassays, there is incomplete understanding of analyte capture parameters. This study presents computational results and corresponding experimental binding assays of analyte capture. Our results identify: 1) a Â¿reagent-limitedÂ¿ regime, under constraints of finite sample volume and assay time; 2) a critical flow rate; 3) an increase in signal by using a short concentrated plug rather than a long dilute plug; 4) a requirement to eventually reach a reaction-limited operating regime to maximize the capture of analytes.

查看译文

关键词

biomems,biochemistry,biomedical measurement,microchannel flow,analyte capture parameters,critical flow rate,experimental binding assays,finite assay time,finite sample volume,microfluidic heterogeneous immunoassays,reaction limited operating regime,reagent limited regime,short concentrated plug,finite element methods,signal processing,fluorescence,solid modeling,signal analysis,testing,flow rate,microchannel,microfluidics

AI 理解论文

溯源树

样例

生成溯源树，研究论文发展脉络

Chat Paper

正在生成论文摘要