Bead-Immobilized Molecular Beacons for High Throughput SNP Genotyping Via a Microfluidic System

Ryan D Sochol,A Mahajerin,Benjamin P Casavant,P K Singh,Megan Dueck,Luke P Lee,Liwei Lin

Sorrento（2009）

引用 5|浏览15

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摘要

This paper reports the first demonstration of single nucleotide polymorphism (SNP) genotyping via molecular beacon probes immobilized on polystyrene microbead substrates within a dynamic microfluidic system. Additionally, we present an optimized bead immobilization technique, micropost array trapping (muPAT), for high-density and high-throughput arraying of beads. Quantitative detection was achieved at room temperature for three label-free DNA oligonucleotide sequences based on the genome of the Hepatitis C Virus (HCV) in humans. SNP detection technology is integral for the realization of personalized medicine and genetic disease identification, and this system offers a promising technique for fast, sensitive, and cost-effective SNP genotyping.

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关键词

dna,biological techniques,diseases,genetics,genomics,microfluidics,microorganisms,molecular biophysics,optimisation,patient treatment,dna oligonucleotide sequences,hepatitis c virus,snp genotyping,bead immobilization,bead immobilized molecular beacons,dynamic microfluidic system,genetic disease identification,micropost array trapping,molecular beacon probes,personalized medicine,polystyrene microbead substrates,single nucleotide polymorphism,molecular beacon,high throughput,biology,cost effectiveness,room temperature,fluorescence

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