Proteome-wide analysis of SUMO2 targets in response to pathological DNA replication stress in human cells.

•SUMO2, but not SUMO1, is conjugated to proteins when human cells are under replication stress.•Proteome-wide analysis of SUMO2 target proteins in response to replication stress performed.•22 proteins found with increased SUMO2 SUMOylation in response to replication stress.•POLD3 is SUMO2 conjugated specifically in conditions promoting common fragile site expression.•POLD3 deficiency leads to an altered stress response.