Preparation of pure, high titer, pseudoinfectious Flavivirus particles by hollow fiber tangential flow filtration and anion exchange chromatography.

•A purification procedure for a replication-deficient flavivirus is described.•Purification is achieved by tangential flow filtration followed by anion exchange chromatography.•The use of a convective monolithic anion exchanger supports recovery of infectious virus.•Purification results in ∼60% recovery of infectious virus titer.•Vaccine virus with titers as high as 1×109FFU/mL have been prepared.