Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation.
CHEMICAL COMMUNICATIONS(2015)
摘要
The ability to rapidly generate a pair of aptamers that bind independently to a protein target would greatly extend their use as reagents for two site ('sandwich') assays. We describe here a method to achieve this through proximity ligation. Using lysozyme as a target we demonstrate that under optimal conditions such a pair of aptamers, with nanomolar affinities, can be generated in a single round.
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关键词
dna aptamers,proximity ligation,binding
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