New insights into the wheat chromosome 4D structure and virtual gene order, revealed by survey pyrosequencing

Marcelo Helguera,Máximo Rivarola,Bernardo Clavijo,Mihaela M. Martis,Leonardo S. Vanzetti,Sergio González,Ingrid Garbus,Phillippe Leroy,Hana Šimková,Miroslav Valárik,Mario Caccamo,Jaroslav Doležel,Klaus F.X. Mayer,Catherine Feuillet,Gabriela Tranquilli,Norma Paniego,Viviana Echenique

Plant Science（2015）

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摘要

•Survey sequence of T. aestivum chromosome 4D was obtained by pyrosequencing.•Near 5700 genes were predicted on 4D chromosome, ∼2200 on 4DS and ∼3500 on 4DL.•A 4D virtual gene order based on synteny with orthologous gene loci is proposed.•Among group 4, higher collinearity exists between 4D and 4B as compared to 4A.•Complementary data to that provided by IWGSC is presented, available at NCBI.

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关键词

Bd,CDS,dN/dS,EST,ISBP,IWGSC,LMP,MDA,NGS,Os,Sb,SE,SSR,SNP

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