Overexpression Of Megsin Induces Mesangial Cell Proliferation And Excretion Of Type Iv Collagen In Vitro

Over-expression of megsin is associated with mesangial cell (MC) proliferation and extracellular matrix (ECM) accumulation. The underlying pathogenesis is unknown. This study demonstrate that overexpression of megsin induced incorporation of [H-3]thymidine in MCs and PDGF-BB, TGF-beta 1 upregulation. Concentrations of PDGF-BB, TGF-beta 1 and type IV collagen in the culture medium of MCs transfected with megsin were higher than controls. Anti-PDGF-BB suppressed incorporation of [H-3]thymidine in MCs transfected with megsin and mRNA expression of TGF-beta 1 in stable transformant MCs, suggesting that over-expression of megsin induces cell proliferation and ECM accumulation in MCs, upregulation of PDGF-BB and TGF-beta 1 is probably the main route involved in pathogenesis. (C) 2011 Elsevier Inc. All rights reserved.