Notice of Retraction In Vivo Study of Adenovirus Mediated Transgenic HIF-1alpha and Its Protective Effects on Cardiac Function - Biochemical, Cellular, Molecular and Tissue Engineering

Notice of Retraction After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE's Publication Principles. We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper. The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org. Aims: Hypoxia inducible factor-1α (HIF-1α) plays important roles in response to hypoxia. In this study, the effects of adenovirus mediated transgenic HIF-1α on cardiac function in acute myocardial infarction (AMI) models was investigated. Methods and Results: Ad-HIF-1α (adenovirus vector containing HIF-1α gene) or Rz-HIF-1α group (adenovirus vector containing HIF-1α ribozyme gene) were transfected into myocardial cells respectively. It was discovered that the expression of HIF-1α and VEGF increased at first, reached the peak at 7d (7th day) and decreased gradually from then on. At 56d (56 day), HIF-1α protein was undetectable, and VEGF protein was hardly expressed as well. Meanwhile, the amount of apoptotic cells was the largest in Rz-HIF-1α group, while the one was rather small in Ad-HIF-1α group. Conclusions: In summary, in animal models of myocardial infarction , this adenovirus mediated medical treatment using Ad-HIF-1α gene injection could reverse both the damage to cardiac function and myocardial cell apoptosis under the hypoxia induction. Significance and Impact of the Study: These results may provide an experimental and theoretical proof of adenovirus mediated transgenic HIF-1α in clinical application of myocardial infarction.