Effects of microbial inoculants on actinomycetes communities diversity during municipal solid waste composting

In order to investigate the effect of microbial enhanced composting on the composting process and actinomycetes communities, the bacterial mixture and fungal compound agents was inoculated to the municipal solid waste pile. The non-inoculated pile was taken as control. The parameters of temperature and wood degradation efficiency of cellulose was measured, a method of PCR-DGGE was used to study the community dynamics and diversity of actinobacteria. The results showed that it could reduce the heating time of composting strengthened by microbe inoculants, and could improve the temperature of the cooling and the secondary fermentation period. Compared to natural composting the degradation rate of hemicelluloses, cellulose and lignin were increased by 8.95%, 12.72% and 10.13%. DGGE profiles showed that the diversity index of the two composting showed significant differences, composting strengthened by microbe inoculants could improve the type and number of dominant microorganisms, and could improve the actinobacteria community diversity during maturity period, which conducive to compost maturity. The results of DNA sequenced were that: Corynebacterium sp, Mycobacterium sp, Streptomyces sp, Thermotoga sp, Dietzia sp, Saccharothrix sp and Actinomyces sp were detected during inoculated compost, they all involved in actinobacteria phylum.