Biocompatibility of electro-spinning beta-tricalcium phosphate/gelatin guided tissue regeneration membrane versus polylactic acid and poly(lactide-co-glycolide) membrane

BACKGROUND: In the past, guided tissue regeneration materials were produced by β-tricalcium phosphate (β-TCP) and gelatin (Gel), but the production of fiber membrane by electro-spinning technique was reported less. OBJECTIVE: To prepare a new kind of Gel with β-TCP hybrid nanofibrous membrane and testing its biocompatibility compared with polylactic acid (PLLA) and poly(lactide-co-glycolide) (PLGA) membrane. METHODS: The β-TCP/Gel guided tissue regeneration membrane was made by electro-spinning technique. Scanning electron microscopy (SEM) was employed to observe membrane surface. MTT test was performed to compare toxicity among β-TCP/Gel, PLLA, and PLGA membranes. RESULTS AND CONCLUSION: The β-TCP/Gel membrane was porous, and β-TCP granules were nodosity-adhered on surface of Gel. The average diameter of fiber was 500-600 nm. The distribution of fiber ranged from 200 to 500 nm. There was no significant difference in toxicity among PLLA, PLGA, and negative control group (P > 0.05). The results suggested that the β-TCP/Gel membrane was low cytotoxicity and suitable for tissue engineering. It would be a promising material for periodontal tissue regeneration.