Identification of major metabolites of 2-hydroxy-1-methoxyaporphine in beagle dogs plasma by liquid chromatography/ion trap mass spectrometry

OBJECTIVE: To investigate the major metabolites of 2-hydroxy-1- methoxyaporphine in Beagle dogs. METHODS: The blood samples were collected from vein after a single dose of 1.5 mg·kg-1 2-hydroxy-1- methoxyaporphine. The plasma samples were purified by a SPE column (SPE-C 18) and analyzed with LC/MSn for potential metabolites. RESULTS: 2-hydroxy-1-methoxyaporphine and its three metabolites were first identified in Beagle dogs plasma. CONCLUSION: The major metabolic pathway of 2-hydroxy-1-methoxyaporphine in Beagle dogs is conjugating with glucuronic acid, forming phase II metabolites.