Determination Of Amino Acids In Root Of Panax Quinquefolius L. By Microwave Hydrolyzation-Derivation-Hight Performance Liquid Chromatography

The high pressure microwave-assisted hydrolyzation was used to hydrolyze the proteins in the root of Panax quinquefolius L. The obtained amino acids were derivated by microwave derivation and determined by HPLC-UV. The conditions in the microwave derivation and hydrolyzation were optimized. The results obtained by microwave hydrolyzation at 190 degrees C, 15 min were similar with that obtained by conventional hydrolyzation. 2,4-Dinitrofluorobenzene (DNFB) was used as derivating reagent. When the derivating time was more than 20 s, the derivating reaction was completed. While conventional derivation reaction took I It at 60 degrees C. The analyzing time of amino acids was greatly reduced. Then the contents of amino acids in the root of Panax quinquefolius L. were determined by HPLC-UV and the results were satisfactory.