Capillary Electrophoresis-Electrochemiluminescence Method For Simultaneous Detection Of Azithromycin, Roxithromycin And Erythromycin Ethylsuccinate

In this paper, a capillary electrophoresis-electrochemiluminescence method for simultaneous detection of three macrolide antibiotics: azithromycin (ATM), roxithromycin (RTM) and erythromycin ethylsuccinate (ETMES) has been presented. The method is based upon the enhancement effect of these compounds on electrochemiluminescence of tris(2,2-bipyridyl) ruthenium(II) (Ru(bpy)(3)(2+)). Under the optimized conditions. complete separation of ATM, WIN and ETMES was achieved within 6 min using 20 mmol L-1 phosphate buffer of pH 7.3 as a background electrolyte and applying a separation voltage of 20 kV. Detection limits were 0.1 mu mol L-1 for ATM, 0.2 mu mol L-1 for WIN, and 0.4 mu mol L-1 for ETMES (S/N = 3). Relative standard deviations for all analytes were below 5%. The proposed method has been successfully applied to the determination of the content of the studied compounds in spiked human plasma and urine samples.