Rapid determination of momordicoside in Momoradica charantia seeds by capillary zone electrophoresis

A novel method is described for the rapid determination of momordicoside in bitter melon based on capillary zone electrophoresis. Electrophoresis conditions, including the pH value and concentration of running buffer, as well as the voltage applied were optimized. The contents of momordicoside in 52 samples originating from different agricultural areas in China were determined. The results showed an excellent linearity in the concentration range of 0.50–2.51 mg mL −1 for all samples ( R 2 > 0.9999), with a recovery of 96.0% and a relative standard deviation of 1.4%. The method is characterized by rapidity, accuracy, the need for small samples only, and is suitable for analyses at a large scale.