Effect of NF-ΚB antisense oligodeoxynucleotides on proliferation of pancreatic carcinoma cells

Objective: To investigate the effect of NF-κB subunit p65 antisense oligodeoxynucleotides(ASODN) on the proliferation of pancreatic carcinoma cells and the therapy effects of p65 ASODN on pancreatic carcinoma. Methods: p65 ASODN were designed and constructed and were transfected into human pancreatic carcinoma cell line PANC-1. The rate of transfection was detected by flow cytometry and fluorescence microscopy. RT-PCR, MTT and clone assay were used to detect the expression level of p65 mRNA and proliferation of PANC-1 cells. Results: p65 ASODN was successfully transfected into PANC-1 cells. After the transfection, RT-PCR revealed that the expressions of p65 mRNA of antisense group, liposomes control group and blank control group were 0.24±0.05, 0.37±0.04 and 0.39±0.03, respectively. The expression of p65 mRNA of the antisense group was significantly lower than those of the other groups, P=0.000. The results from the MTT assay indicated that 24 hours, 48 hours and 72 hours after the transfection, the cell proliferation of the antisense group was inhibited. The inhibition peaked at 48 hours after the transfection, P<0.01. The clone assay revealed that the cloning efficiencies of the antisense group, liposomes control group and blank control group were (14.9±3.3)%, (23.3±2.0)% and (25.8±2.5)%. The cloning efficiency of the antisense group was reduced, P=0.000. Conclusions: The transfection of p65 ASODN could effectively down-regulate the expression of p65 mRNA and reduce cell proliferation of PANC-1 cells. The therapeutic prospect of p65 ASODN on pancreatic carcinoma is worthy of further investigation.