A Novel Developed Detection And Analysis Method Of Dna Microarray Hybridization Using Fret Technique

Fluorescence Resonance Energy Transfer (FRET) is a distance-sensitive energy transfer process which is widely used in probing molecular interaction in the 1-10 nm range. DNA chip technique is a high throughput analysis method in molecular biology research. In this paper, we explored a novel detection and analysis method of DNA microarray hybridization using FRET technique. In our study, TMR dye labeled DNA oligomer was immobilized on the microarray chips and hybridized with complementary DNA oligomer labeled with Cy5 dye. We successfully detected and analysed the FRET signal of the hybridized DNA microarray. The variation of FRET signal intensity and the efficiency of FRET in response to the concentration of the sample were studied.