Headspace Solid-Phase Microextraction-Gas Chromatography-Mass Spectrometry For Analysis Of Volatile Components From Atractiodes Macrocephala Koidz

Headspace solid-phase microextraction HS-SPME technique was employed to extract the volatile components from Chinese traditional medicine Atractlodes macrocephala Koidz. The volatile components were isolated and identified successfully by gas chromatography-mass spectrometry GC-MS. The results from HS-SPME-GC-MS were compared with those obtained from steam distillation-GC-MS SD-GC-MS with a good agreement. The volatiles were collected using a polydimethylsiloxane-divinylbenzene PDMS-DVB fiber by HS-SPME. The best response was obtained when the extraction temperature was 70 degrees C the equilibrium time and extraction time were all 30 min and the desorption time was 4 min. Analysis was performed by GC-MS with a polysiloxane capillary column 30 m x 0. 25 mm film thickness 0. 25 mu m using He as the carrier gas and a programmed temperature run. Forty-one components accounting for 90. 810% were identified. The main components relative amount more than 1% of the samples by HS-SPME were atractylone 40. 12% gamma-elemene 14.73% aromadendrene 13. 05% eudesma-4 14 11-diene 5.46% caryophyllene 2.56% patchoulene 2.55% 6 10 11 11-tetramethyl-tricyclo 6.3.0.1 2 3 undec-7-ene 2. 11% cedrene 1.48% alpha-caryophyllene 1.48% and selina-4 14 -7 11 diene-8-one 1.01%. By SD-GC-MS 31 components accounting for 88. 19% were identified and all these components could be extracted by SPME except trans-beta-ocimene which accounts only 0. 10%. The results showed that the HS-SPME technique can be used to extract the the volatile components from Atractlodes macrocephala Koidz. in place of the traditional time-consuming SD.