Interfacial construction of gold nanoshells on 3-aminopropyl triethoxysilane modified ITO electrode surface for studying cytochrome b 562 electrochemistry

An interface of gold nanoshells (GNSs) was constructed on the surface of the 3-aminopropyl triethoxysilane (APTES) modified ITO glass substrates by a simple self-assemble method to form the GNSs-coated ITO electrode. UV-vis spectroscopy, scanning electron microscopy (SEM), and cyclic voltammetry were used to characterize the GNSs interface architectures. SEM and UV-vis spectroscopy showed that an interconnected and stable GNSs interface was formed on the APTES modified ITO glass substrate. The cytochrome b(562) (Cyt b(562)) was selected to observe electron transfer reactions of redox protein at the GNSs-coated ITO electrodes. Quasi-reversible electrochemistry of Cyt b(562) was obtained and its electrochemical behaviors were discussed.