Determination of tropisetron in human plasma by liquid chromatography tandem mass spectrometry

A fast and sensitive liquid chromatography tandem mass spectrometric method has been developed and validated for the determination of tropisetron in human plasma. The HPLC separation was performed on a Phenomenex Synergi Fusion RP80 column using acetonitrile -13mM ammonium acetate - acetic acid (30:70:0.035, v/v) as the isocratic mobile phase. The assay was linear over the concentration range 0.5-128ng/mL. The intra- and inter-assay precision was less than 11.6% for tropisetron. The method was successfully used to characterize the pharmacokinetic profiles of tropisetron in 20 healthy volunteers after an intravenous infusion of 5mg tropisetron.