Direct Observation of Non-covalent Complexes Formed Through Phosphorylated Flavonoid Protein Interaction by Electrospray Ionization Mass Spectrometry

Chen XL, Qu LB, Zhang T, Liu HX,Yu F,Yu YZ, Zhao YF

SUPRAMOLECULAR CHEMISTRY（2004）

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摘要

7-Hydroxyflavone was phosphorylated by a modified Atherton-Todd reaction and the resulting structure determined by NMR, ESI-MS/MS and elemental analysis. The electrospray ionization results showed that phosphorylated flavonoids can form non-covalent complexes with many proteins such as lysozyme, cytochrome c, bovine insulin and myoglobin, while non-covalent complexes were not detected with a mixed solution of 7-hydroxyflavone and proteins. It is deduced that phosphorylated flavonoids possess relatively strong affinities for proteins and form non-covalent complexes with proteins more easily than the unphosphorylated compounds.

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关键词

7-hydroxytlavone,phosphorylation,myoglobin,DEPH,ESI-MS,lysozyme,cytochrome c,bovine insulin,non-covalent complexes

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