Rayleigh Light-Scattering System Of Manganese-Tetrasulfonatophthalocyanine-Proteins And Its Application

At pH 3.6, the interaction of manganese-tetrasulfonatophthalocyanine with proteins results in enhanced Rayleigh light scattering signals at 344 nm. Proteins including bovine serum albumin, human serum albumin, human gamma-immunoglobulin, and ovalbumin, can be determined under optimum conditions', the,linear ranges of the calibration curves were 0.0similar to1.6 mg/L for human serum albumin, 0.05similar to2.0 mg/L for human gamma-IgG, 0.1similar to3.0 mg/L for the bovine serum albumin and 0.0similar to1.0 mg/L for ovalbumin, the corresponding detection limits were 5. 7, 8.3, 13 and 13 mug/L, respectively. The method has been applied-to the analysis of total protein in human serum samples collected from the hospital and the results are satisfactory.