Analysis Of Amino Acids In A Single Human Red Blood Cell By Capillary Zone Electrophoresis With Intracellular Nda-Derivatization And Electrochemical Detection

A novel method for determination of amino acids in individual human red blood cells has been developed. In this method, the derivatization reagents (NDA and CN-) are introduced into living cells by electroporation. After completion of derivatization, the amino acids in a single cell is determined by capillary zone electrophoresis with end-column amperometric detection.