Effects of anisodamine against myocardial ischemia-reperfused injuries and antilipid peroxidation

Aim: Anisodamine (Ani), an alkaloid first isolated in China. To study the relationship between the protective effects of Ani on myodardial cells of reperfused injuries and the antilipid peroxidation. Methods: Coronary ligation for 15-min followed by 10 min reperfusion was performed in anesthetized rats. Results: Ani 1, 3, 5 mg �� kg-1 iv 1 min prior to reperfusion could dose-dependently lower the release of creatine kinase (282 �� 29, 252 �� 53, 226 �� 50), counteract the increase of malondialdehyde content (3.3 �� 1.3, 3.2 �� 1.6, 3.1 �� 1.2) in the reperfused myocardium and preserve the SOD activity (41 �� 7, 46 �� 8, 55 �� 8). Ani completely abolished the drop in the contents of principal unsaturated fatty acids (oleic, linoleic, and arachidonic acids) of the membrane lipids in the reperfused myocardium. SOD 75 U �� kg-1 iv 1 min prior to reperfusion exerted similar effects like Ani 3 mg �� kg-1. Conclusion: It is suggested that the antilipid peroxidative effect of Ani may contribute to its protection against reperfusion-induced myocardial injuries.