Study on triterpenoid and flavonoid contents of Rabdosia japonica

Objective To extract and identify the chemical constituents of Rabdosia japonica. Methods The constituents were isolated and purified by chromatography on silica gel, Sephadex LH-20 and HPLC. The structures of the compounds were determinedbymass spectrometry (MS) and nuclear magnetic resonance (NMR) spectra1 analysis. Results Totally 10 compoundswere isolated and their structures were identified as four triterpenoids: oleanolic acid (1), ursolic acid (2), 2α-hydro xyursolic acid (3), 2α, 23-dihydroxyursolic acid (4) - six flavonoids: quercetin (5), apiginin (6), luteolin (7), rutin, (18) isorhamnetin (9), and tricin (10). Conclusion Isorhamnetin (9) and tricin (10) had been isolated from this plant for the first time.