Identification of the differentially expressed genes in SP2/0 myeloma cells from Balb/c mice infected with Trichinella spiralis.

To study the molecular mechanism of suppressed growth caused by Trichinella spiralis, an SP2/0 myeloma model was established using parasite-infected Balb/c mice. Suppression subtractive hybridization (SSH) was then utilized to identify differentially expressed genes between tumor cells from the infected and non-infected mice. On the 11th day after infection, 2×106 SP2/0 myeloma cells were subcutaneously inoculated into 6–8 week old female Balb/c mice in both the experimental and control groups. Twenty-eight days after tumor cell inoculation, the mice were euthanized and the sizes and weights of the tumors were measured. Messenger RNA was isolated and used to perform SSH. Putative differentially expressed genes were identified, sequenced and analyzed by BLASTn. Among the sequences detected which ranged in size between 180 and 850bp, genes encoding RpL41, NKTR, Rbbp4 and ANXA2 were enriched and considered possible proteins involved in tumor growth inhibition.