Production and characterization of mouse monoclonal antibodies against lysis protein E of phiX174.

Shenye Yu,Hailing Zhao,Huanan Wang,Xiumei Wang,Guangxi Shao,Lili Xu,Wei Si,Liping Chen,Wanjiang Zhang,Siguo Liu

Journal of Virological Methods（2013）

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摘要

•Purified mAbs were screened against a Ph.D.-12 library.•One epitope showed a good match with protein E at 57–63aa (KPLN--R).•Epep (VRLKPLNCSR) strongly inhibited combination of E-A5 with E fusion proteins.•E-A5 indicated specific immunoreactivity with E. coli-expressed native protein E.

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关键词

Monoclonal antibody,Phage phiX174,Protein E,Epitope

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