One-pot preparation of uricase-poly(thiophene-3-boronic acid)-Pt nano composites for high-performance amperometric biosensing of uric acid

Chemical oxidation of glycoprotein-bound thiophene-3-boronic acid (TBA) monomer by relatively mild oxidant chloroplatinate in neutral aqueous solution containing uricase yielded uricase–PTBA–Ptnano bionanocomposite with high enzymatic specific activity on a platinized (Ptplate) Au electrode, which was then covered by an outer-layer chitosan (CS) film to fabricate a CS/uricase–PTBA–Ptnano/Ptplate/Au electrode. This electrode exhibited a linear amperometric response to uric acid concentration from 5μM to 1.2mM with a sensitivity of 134μAmM−1cm−2, a limit of detection (S/N=3) of 1μM, and excellent operation/storage stability, which also worked well in serum samples.