A proteomic analysis of larval midguts of Boettcherisca peregrina in response to cadmium exposure

In this study, the changes of protein expression in larval midgut cells of Boettcherisca peregrina Robineau-Desvoidy (Diptera Sarcophagidae) after exposure to 800 mu g cadmium (Cd) per g diet for 48 h were investigated by two-dimensional polyacrylamide gel electrophoresis (2DE) plus with mass spectrometric analysis. The results showed the expression of ten proteins of midgut cells increased in response to acute Cd stress. Of these, nine were successfully identified, and seven of them are relevant to cellular detoxification processes. Functions of these seven proteins include removal of Cd (P-glycoprotein, P-gp), stress response (Heat shock protein, Hsp), osmoregulation and metal ion transport (Cd-inducible lysosomal protein CDR). This study will provide valuable information for the cell defense mechanism against heavy metals.