Expression and clinical significance of von Hippel-Lindau downstream genes: Jade-1 and β-catenin related to renal cell carcinoma.

OBJECTIVE To investigate the expression of Jade-1 and beta-catenin and their effect in the development of renal cell carcinoma (RCC). METHODS The expression of Jade-1 and beta-catenin in 11 normal kidney tissue specimens (normal group) and 60 RCC specimens (RCC group) was determined using real-time polymerase chain reaction and immunohistochemistry. Also, their effect on early relapses of RCC was analyzed after 5 years of follow-up. RESULTS The expression of Jade-1 protein in the RCC group was significantly lower than that in the normal group (0.1655 vs 0.7438, P < .05), and the expression of beta-catenin protein was significantly greater than that in the normal group (0.2756 vs 0.0855, P < .05). The expression of Jade-1 mRNA in the RCC group was 0.202 times that in the normal group, which were lower (P < .05). The expression of beta-catenin mRNA was 1.014 times that in the normal group (P < .05). The expression of Jade-1 protein and beta-catenin protein in poorly differentiated RCC specimens was significantly lower and higher than the expression in the well-differentiated RCC specimen (P < .05), respectively. Patients with negative Jade-1 expression and positive beta-catenin expression were found to have shorter survival on univariate analysis (P < .05). CONCLUSION RCC with a low expression of Jade-1 and high expression of beta-catenin is associated with a poor outcome and decreased survival. UROLOGY 80: 485. e7-485.e13, 2012. (c) 2012 Published by Elsevier Inc.