Simultaneous Analysis Of Three Avermectins In Soils By High-Performance Liquid Chromatography With Fluorescence Detection

A simple and sensitive method has been developed and validated for the determination of abamectin B-1a (ABA B-1a), emamectin B-1a (EMA B-1a) benzoate and ivermectin H2B1a (IVM H2B1a) in soils. The avermectins (AVMs) residues were extracted from soils with acetonitrile/water (9 : 1, v/v) and then were purified on C-18 solid-phase extraction (SPE) cartridge. After being derivatised by N-methylimidazole (N-MIM) and trifluoroacetic anhydride (TFAA), the residues of three AVMs were analysed by high-performance liquid chromatography with fluorescence detection (HPLC-FLD). The method was validated in terms of system suitability, linearity, selectivity, precision, recovery, specificity and stability. There was a good linear relationship (R-2 > 0.99) for three AVMs ranged from 0.01 to 5 mu gmL(-1). The LOD and LOQs of ABA B-1a, EMA B-1a benzoate and IVM H2B1a for standard solutions were 1.1-1.7 and 3.6-5.7 mu gL(-1) respectively. The accuracy of AVMs in soils was from 83.7 to 115.5% with precision less than or equal to 12.4%. Using the developed method, 9 soil samples with 9.3-12806.3 mu gkg(-1) of AVMs residues had been detected.