A rapid microtiter plate assay for measuring the effect of compounds on Staphylococcus aureus membrane potential

Journal of Microbiological Methods, pp. 254-256, 2010.

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Keywords:
Membrane potentialStaphylococcus aureusMicrotiterAssay

Abstract:

We developed a homogenous microtiter based assay using the cationic dye 3, 3′-Diethyloxacarbocyanine iodide, DiOC2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activity due to unknown modes of action at le...More

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