A recombinant adenovirus expressing the e2 protein of classical swine fever virus protects pigs from lethal challenge
Chinese Journal of Animal Infectious Diseases(2009)
摘要
Classical swine fever(CSF),which is caused by classical swine fever virus(CSFV),causes significant losses in pig industry in many Asian and European countries.The E2 glycoprotein of CSFV is the main target for neutralizing antibodies.We have evaluated previously the recombinant adenovirus(rAdV-E2) expressing the E2 glycoprotein of classical swine fever virus(CSFV) in rabbits.The results showed that the rabbits immunized with rAdV-E2 developed high-level CSFV-specific antibodies and were all free of the regular fever and the viral replication in the spleen upon challenge with C-strain,which were seen in the rabbits immunized with the parent adenovirus of rAdV-E2.This study was designed to evaluate further the efficacy of rAdV-E2 in pig.Pigs were immunized twice with 108 TCID50 of rAdV-E2(n = 5) or control wtAdV(n = 5),respectively,and challenged with virulent Shimen strain at 9 weeks of post boost following the booster immunization.The results indicated that pigs immunized with rAdV-E2 developed high titers of specific neutralizing antibodies against CSFV after the booster,and the antibody titers increased rapidly upon challenge.The immunized pigs showed no other clinical symptoms except short-term fever,whereas,the control pigs immunized with wtAdV produced no detectable antibody before challenge,and showed apparently clinical signs after challenge.All control animals developed extended viremia as detected by real-time RT-PCR.Severe pathologic lesions typical of CSFV infection were observed at necropsy.It is concluded that the recombinant adenovirus rAdV-E2 might be a vaccine candidate against CSFV.
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关键词
immunogenicity,recombinant adenovirus,E2 gene,pig,Classical swine fever virus
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