P124. 3-Polyunsaturated fatty acids may inhibit angiogenesis through suppression of ELR+ CXC chemokines expression and VEGF signaling in pancreatic cancer
DIFFERENTIATION(2010)
摘要
Angiogenesis, the process of new blood vessel formation, is essential for tumor growth and metastasis. Although the protective role of ω3-PUFAs on cancer progression has been reported, the anti-angiogenic mechanisms remain to be further understood. The aim of this study is to investigate the anti-angiogenic mechanisms of ω3-PUFAs in pancreatic cancer. In angiogenesis protein array chip assay, only angiogenic ELR+ CXC chemokines expression, which activate endothelial cell migration and neutrophils recruitment, was significantly reduced by DHA treatment in SW1990 human pancreatic cancer cells. The reduced expression of ELR+ CXC chemokine was confirmed by RT-PCR. Chemotactic migration of HUVEC was significantly reduced in DHA-treated SW1990 conditioned medium (CM) compared to control CM. DHA also inhibited VEGF reporter activity in SW1990 cells. VEGF-induced endothelial cell proliferation and migration were markedly inhibited by DHA treatment. MMP-2 and MMP-9 promoter activities were reduced by DHA treatment, and ultimately attenuated the invasiveness of SW1990 cells as revealed by matrigel droplet assay. Moreover, NF-κB reporter activity, which is closely correlated with the expression of CXC chemokine, VEGF and MMPs, was reduced by DHA treatment. When mouse pancreatic cancer PANC02 cells were implanted into Fat-1 transgenic mice, tumor size and volume were dramatically reduced in Fat-1 mice compared to control mice. In immunohistochemical analysis of tumors of Fat-1 mice, apoptosis (TUNEL positive cells) was markedly increased and microvessel density (CD31 intensity) was significantly reduced in Fat-1 mice compared to control mice. In addition, VEGF-induced neovascularization to a subcutaneous matrigel plug was significantly inhibited in Fat-1 mice compared with control mice. Our finding suggests that ω3-PUFAs can suppress tumor angiogenesis through inhibition of ELR+ chemokines expression and VEGF signal in pancreatic cancer. Thus ω3-PUFAs supplementation may beneficial for chemoprevention and treatment of pancreatic cancer by anti-angiogenic effect. This work was supported by basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (R13-2007-020-01000-0 and 2010-0016447), Korea.
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关键词
polyunsaturated fatty acid
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