Using active microfluidic flow focusing to sort particles and cells based on high-resolution volume measurements

•We combine a high-resolution resistive pulse sensor with hydrodynamic flow focusing.•We quantify sorting purity, throughput and resolution with polystyrene microspheres.•We size/sort a population of Saccharomyces cerevisiae (yeast) cells.•We integrate PDMS valves, both for sensitivity enhancement and cell collection.