Cellular solid-phase binding assay and mass spectrometry for screening of α4β7 integrin antagonists

A qualitative cellular solid-phase binding assay for screening α4β7 integrin antagonists attached via photolinker to TentaGel® Macrobeads has been developed. An activation of the integrins with Mn2+ was necessary to achieve binding to the bead bound antagonists. The identification of the resin bound compounds was done by mass spectrometry.