Genetic relationships among ginger accessions based on AFLP marker Kekerabatan antar nomor-nomor aksesi jahe berdasarkan marka AFLP
msra
摘要
Ginger (Zingiber officinale Rosc.) is a rhizomatous perennial herb and one of the important crop of the genus Zingiber. There are three types of ginger in Indonesia, i.e. big ginger, small ginger, and red ginger. Their rhizomes differ in shape, color, aroma, and chemical composition. To understand the genetic relationships among the three types of ginger, 28 accessions consisted of 22 Indonesian cultivated ginger and 3 accessions each of African and Japanese commercial ginger were analyzed. Total DNA was extracted from rhizome using cetyltrimethyl ammonium bromide (CTAB) method then purified by magnetic beads. Amplified fragment length polymorphism (AFLP) was carried out according to the protocol described in AFLP TM plant mapping kit (PE Applied Biosystem) and the final polymerase chain reaction (PCR) products were separated on 5% denatured polyacrylamide gel on an ABI 373 sequencer. The number of fragments produced by a primer combination of AFLP ranged from 47 to 137 with an average of 96. A total of 221 polymorphic bands were ob- served by using 21 selective primer combinations. Dendrogram based on unweighted pair group methods of arithmetic average (UPGMA) revealed that the gingers could be classified into three major clusters. The red ginger was genetically far from the big ginger, but close to some accessions of small ginger. There was no clear genetic differentiation between the small and big types of ginger. The genetic diversity of small ginger (Ht = 0.25) is higher than that of the big ginger (Ht = 0.08).
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关键词
zingiber officinale,ginger,aflp,genetic varia- tion
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