Transcription Initiation Is Localized to a TATA-less Region in the Ovine β1 Adrenergic Receptor Gene

We cloned and sequenced the ovine beta, adrenergic receptor (beta(1)AR) gene and promoter region. The transcription start site was localized by RNase protection and primer extension to a GC-rich region. The predominant initiation sequence did not resemble an initiator element and there was no upstream TATA box. Sequence analysis revealed several potential thyroid hormone and glucocorticoid regulatory elements. Identification of the promoter structure of this important gene will help define its novel regulation during development. (C) 1995 Academic press, Inc.