Activation of chicken adipose tissue diglyceride lipase by cyclic AMP-dependent protein kinase and its deactivation by purified protein phosphatase
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS(1978)
摘要
Diglyceride lipase of chicken adipose tissue was found to be activated by cyclic AMP-dependent protein kinase to the same extent as hormone-sensitive triglyceride lipase (3-to 10-fold) when lipase assays were carried out in buffers of low ionic strength. Sodium phosphate (50 mM) or sodium chloride (100 mM) preferentially enhanced the basal (nonactivated) form of diglyceride lipase, which minimized the apparent activation by protein kinase. The activated diglyceride lipase was readily deactivated by a pure protein phosphatase from bovine heart (MW 35,000) and the deactivated enzyme was then reactivated by protein kinase.
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关键词
protein kinase,adipose tissue,protein phosphatase,cyclic amp
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