Developmental-Changes Of Latency And Udp-N-Acetylglucosa Mine (Nag) Effect On Hepatic Udp-Glucuronyltransferase (Udp-Gt) In Rat-Liver Intact Microsomes

Membrane bound-luminally oriented hepatic UDP-GT is largely unexpressed in liver intact microsomes, and is fully revealed by pretreatment with membrane perturbing agents (latency). Whether the response of UDP -GT to these agents changes during development is poorly known, besides the few existing studies having been carried out without monitoring of microsomal structural integrity. We examined the response of UDP-GT (naphtol as substrate) to a detergent (CHAPSO) and to the putative physiological cofactor NAG in developing rat liver microsomes. Phospholipid (PL) membrane content, and 2 independent parameters of membrane integrity, i.e. latency of low Km microsomal marker Man6Pase and permeability to EDTA, were also assessed.