Influence Of 17 Beta-Estradiol Binding By Dissolved Organic Matter Isolated From Wastewater Effluent On Estrogenic Activity
Ecotoxicology and environmental safety(2011)
摘要
The aims of this study were to determine the sorption coefficient (Log K(DOC)) of 17 beta-estradiol (E2), according to the size and composition of dissolved organic matter (DOM) isolated from wastewater effluent using a fluorescence quenching (FQ) method, and to measure the estrogenic changes due to the extent of E2 sorption onto effluent DOM (EfOM) by using an E-screen bioassay. The highest log K(DOC) of E2 for a DOM size fraction less than 0.2 mu m was observed by 4.87 +/- 1.87 L kg(DOC)(-1), and its estrogenicity was the lowest among all the size fractions at 1.2 ng-EEQ L(-1). However, E2 estrogenicity for a DOM size fraction less than 5 kDa was as high as that for the positive control due to the absence of fulvic acid- and protein-like DOM to bind with E2. The estrogenic activity for the hydrophobic fraction bound with E2 was significantly reduced to 1.6 ng-EEQL1, while that for the hydrophilic fraction having no binding with E2 was 2.6 ng-EEQ L(-1), which was similar to that of the positive control (i.e., 2.8 ng-EEQ L(-1)). The results support a conclusion that the change in estrogenicity was due to the strong sorption affinity of E2 onto DOM. (C) 2011 Elsevier Inc. All rights reserved.
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关键词
17 beta-Estradiol (E2),Effluent dissolved organic matter,Sorption,Estrogenicity
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