Analysis of Dyslipidemia in Nephrotic Syndrome by Capillary Isotachophoresis

Objective. Capillary isotachophoresis (cITP) is an effective way to analyze serum lipoprotein owing to high sensitivity and resolution. Serum lipoproteins can be separated into 9 subpopulations by cITP in 6 min. Lipoprotein data obtained by cITP were compared with those obtained by the routine method. Correlation coefficient was 0.86 for HDL-C and 0,83 for LDL-C (both P<0.001). Furthermore, slow and fast-migrating high-density lipoprotein (HDL) in the cITP lipoprotein pattern allowed for a rough estimation of lecithin-cholesterol acyltransferase (LCAT) activity. The ratio between chylomicrons (CM) and intermediate density lipoprotein (IDL), and between IDL and LDL could be used to predict lipoprotein lipase (LPL) and hepatic triglyceride lipase (HL) activity, respectively. Compared to the control group, the ratios of sHDL/fHDL, CM/IDL, and IDL/LDL in the nephrotic syndrome (NS) group were significantly increased, while LCAT, LPL, and HL activities were significantly decreased and negatively correlated with these ratios. Conclusion: Capillary isotachophoresis would prove to be a novel and effective method for analyzing the composition and quantitation of lipoproteins and predicting lipolytic enzyme activity.