Development of an immunochromatographic strip test for the rapid detection of deoxynivalenol in wheat and maize

An anti-DON monoclonal antibody (Mab) was produced from a stable hybridoma cell line, 12D1, generated by the fusion of SP2/O myeloma cells with spleen cells isolated from a Balb/c mouse immunized with DON–cationic bovine serum albumin (CBSA) conjugate. The 12D1 Mab belongs to the immunoglobulin G1 (κ-chain) isotype.