The Pure Cultivation of Tricholoma matsutake and It’s ITS Identification

In this study,the mycelia of Tricholoma matsutake from Zhongdian,Lijiang,Wuding and Weishan were cultivated by the method of tissue separation and identified by the method of sense organ distinguishing and ITS sequencing.The results showed that the mycelia of maternal strain were well induced and the rate of induction is 75% on the slant medium of PDA+ yeast extract paste(0.5%).The mycelia gained above could grow very well and be cultivated in larger scale on liquid culture medium.The mycelia of liquid culture had special smell as which Tricholoma matsutake had,and this showed it were likely to be pure culture from Tricholoma matsutake.By ITS sequencing,the genetic identities,between mycelia of liquid culture and fruiting body from Zhongdian,Lijiang,Wuding and Weishan,were 0.997,1.00,0.998 and 0.998 respectively,that implied the mycelia were indeed from their corresponding fruiting body.