A new method of lodinating collagens for use in radioimmunoassay
Analytical Biochemistry(1979)
摘要
Purified collagens from a variety of species were iodinated to a high specific activity with the N-hydroxysuccinimide ester of I125-labeled p-hydroxyphenyl propionic acid (Bolton-Hunter reagent). Labeling had no effect on the immunoreactivity of the collagen as determined by hemagglutination inhibition. This compound presumably acylates the abundant ϵ-amino groups of lysyl and hydroxylysyl residues in the collagen molecule. Using this method it is possible to label pepsin-extracted collagen from which the terminal nonhelical extensions containing tyrosine have been cleaved. The application of Bolton-Hunter-labeled collagens to radioimmunoassay of affinity-purfied antibodies against collagen is demonstrated.
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关键词
hemagglutination inhibition,specific activity,amino acid,radioimmunoassay,nuclear medicine,radiation chemistry,chemical reaction
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