α-Mannosidase in human red cells

1.1. A search for lysosomal hydrolases and related enzymes has been made in hemolysates from human and rabbit red cells. Apart from acid phosphatases, significant activities were found only for α-mannosidase, neutral α-glucosidase and β-hexosaminidase.2.2. α-Mannosidase (α-d-mannoside mannohydrolase, EC 3.2.1.24) activity per cell in human red blood cells was 200-times lower than in white cells, The optimal pH was 5.5–6.0. Electrophoresis on cellulose acetate showed three bands.