Evaluation of fibrin glue prepared by recycled cryoprecipitation in experimental liver surgery

Fibrin glue (FG) is a two component biologic system with adhesive, sealant and topical hemostatic properties, containing fibrinogen, factor XIII, fibronectin, thrombin, some antifibrinolytic agent and ionized calcium. The purpose of the study was to evaluate the hemostatic effectiveness of FG prepared by a modification of cryoprecipitation technique in experimental rat liver surgery. FG component 1 was prepared by triple or “recycled” cryoprecipitation method from single-donor plasma. Rats subjected to liver incision, partial and total lobectomy were treated with FG on the surgical cut surface or underwent standard surgical technique and the efficacy of the FG-treatment was evaluated on the basis of the 24-hour survival ratio and peripheral blood hematological parameters. The mean values of fibrinogen, FXIII, fibronectin and horizontal tensile strength of FG were 54.2 ± 19.9 g/l, 8.03 ± 2.3 IU/ml, 3103.1 ± 148.9 mg/l, and 1.076 ± 0.18 N/cm 2 , respectively. The survival of FG-treated rats subjected to partial and total lobectomy was significantly higher in comparison to the FG-nontreated animals, accompanied with higher values of red blood cell counts, hemoglobin concentration, as well as hematocrit. The survival of animals subjected to liver incision was not significantly different, however the differences in hematological parameters were significant in favor FG-treated animals. The presented results demonstrated that using “recycled” cryoprecipitation it is possible to obtain high quality single-donor FG with successful hemostatic therapeutical effects, as confirmed in the experimental rat liver surgery.