Hydrogen Peroxide Oxidation of Phosphite Triesters in Oligonucleotide Syntheses

The oxidation of P(III) phosphite triesters to stable P(V) phosphates in oligonucleotide Syntheses using hydrogen peroxide was demonstrated in neutral aqueous acetonitrile as a cheap, green alternative to the widely used iodine pyridine procedure. This oxidation was utilized on short oligomers of two nucleosides.