Generation and identification of four new monosomic addition lines of flowering Chinese cabbage-Chinese kale

A selection test of four new flowering Chinese cabbage ( Brassica campestris L. var. utilis Tsen et Lee)-Chinese kale ( Brassica alboglabra Bailey) monosomic alien addition lines was conducted by karyotype analysis and morphological marker identification. The results mainly showed the following: 61‘2 n + 1’ plants screened out from 655 progeny plants of the trigenomic hybrid (AAC) backcrossed with its parent flowering Chinese cabbage (AA), four new monosomic alien addition lines(AA + C 1 , AA+ C 5 , AA+ C 8 and AA + C 9 ) obtained from the 61 ‘2 n + 1’ plants, the transmission rates of the monosomic alien addition lines (AA + C 1 , AA+C 5 and AA + C 8 ) of 19.7%, 23.44%, and 36.51% by female gametes and 7.84%, 8.89%, and 9.43% by male gametes, respectively, and the alien chromosome C-1 and C-5 had partial homology with at least one chromosome of A-genome.